GCSE Biology – Production of insulin

Last updated: 03/07/2020
CCEA GCSE Science – Combined science higher Genome, chromosomes, DNA and genetics GCSE Biology – Production of insulin

Learning Objectives

-I can describe the basic techniques used to produce human insulin
-I can describe the need for restriction enzymes and down streaming

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  3. Answered

  1. What is needed to produce insulin via genetic engineering?

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  2. What is the definition of a restriction enzyme?

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  3. How is the human insulin gene removed?

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  4. What else is the restriction enzyme used to cut open?

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  5. What do restriction enzymes leave?

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  6. What are sticky ends?

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  7. How are the sticky ends from the human DNA and bacterial plasmid joined together?

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  8. What is used to join the insulin gene and the bacterial plasmid?

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  9. Where is the bacterial plasmid containing the insulin placed?

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  10. Where is the bacterial cell placed?

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  11. What process occurs in the fermenter?

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  12. What are the conditions needed for the fermenter?

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  13. What are the conditions needed for the fermenter?

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  14. What process then occurs?

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  15. What is the definition of downstreaming?

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  16. What is insulin used to treat?

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