A Level biology – Optical microscopes

Last updated: 17/02/2022

AQA A-Level Biology Cell structure A Level biology – Optical microscopes

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Question 1 of 19

1. Question
What is the resolution of an optical microscope ultimately limited by?
- The wavelength of light and the distance between two objects
- The shape of the lenses in the microscope
- The brightness of light shining on a sample
- The distance between the objective and eyepiece lenses
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Question 2 of 19

2. Question
Beyond the limit of resolution, increasing the magnification will…
- Reveal no more detail
- Reveal more detail
- Increase the distance between the objects being imaged
- Decrease the distance between the objects being imaged
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Question 3 of 19

3. Question
Why is a light microscope not usually used to magnify greater than 1000x ?
- There is no further increase in resolution
- Lenses cannot magnify any more than that
- There is no need to magnify more
- The wavelength of light is too short
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Question 4 of 19

4. Question
A compound microscope is a type of what?
- Optical microscope
- Transmission electron microscope
- Scanning electon microscope
- Simple microscope
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Question 5 of 19

5. Question
Some early microscopists claimed to see an homunculus or tiny man in nuclei and sperm cells. Apart from their bias and imagination what may have lead to these observations?
- Artefacts
- Contamination
- High resolution
- Miscalculation and magnification
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Question 6 of 19

6. Question
Which type of radiation is used in a compound microscope to image an object?
- Visible light
- X-ray
- Microwave
- Electron-beam
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Question 7 of 19

7. Question
An object being imaged with an optical microscope is usually mounted on what?
- A slide
- A lens
- A stage
- A grid
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Question 8 of 19

8. Question
Which of the following is a limitation of the light microscope?
- It cannot see detailed structure of organelles
- It can visualise living tissue
- It can see detailed structure of bacteria
- It’s resolution is higher than electron microscopes
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Question 9 of 19

9. Question
Which of the following is a benefit of the light microscope?
- It can image living samples
- It can resolve individual protein complexes
- It can reveal detail structure of organelles
- It can be used to image viruses
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Question 10 of 19

10. Question
The magnifying power of a microscope is found by
- Objective lens magnification multiplied by eyepiece lens magnification
- Objective lens magnification divided by eyepiece lens magnification
- Reading the objective lens magnification
- Reading the eyepiece lens magnification
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Question 11 of 19

11. Question
The order that light passes through a microscope’s parts is:
- Condenser, sample, objective lens, eyepiece lens
- Eyepiece lens, sample, objective lens, condenser
- Condenser, sample, eyepiece lens, objective lens
- Sample, condenser, objective lens, eyepiece lens
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Question 12 of 19

12. Question
The ability to distinguish between two points on an image is called
- Resolution
- Magnification
- Focus
- Staining
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Question 13 of 19

13. Question
How many times bigger an image is compared to an object is referred to as what?
- Magnification
- Resolution
- Focus
- Wavelength
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Question 14 of 19

14. Question
The lenses used in an optical microscope are what shape?
- Convex
- Concave
- Contour
- Contrail
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Question 15 of 19

15. Question
The wavelength of light means that optical microscopes can only distinguish between two objects if they are how far apart?
- 0.2 µm or greater
- 0.2 cm or greater
- 0.2 mm or greater
- 0.2 nm or greater
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Question 16 of 19

16. Question
How many lenses are present in a compound microscope?
- Two
- One
- Three
- Four
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Question 17 of 19

17. Question
Put the following units of length in order from the shortest to longest. 1. Millimetre 2. Nanometre 3. Micrometre 4. Centimetre
- 2-3-1-4
- 3-2-1-4
- 3-1-2-4
- 2-3-4-1
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Question 18 of 19

18. Question
Which of the following could not be resolved with an optical microscope?
- An individal ribosome
- An individual bacterium
- An individual red blood cell
- An individual hair
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Question 19 of 19

19. Question
Which of the following could be resolved with an optical microscope?
- An individual bacterium
- An individual ribosome
- An individual virus
- An individual plasmid
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