A Level biology – Electron microscopes

Last updated: 17/02/2022

AQA A-Level Biology Cell structure A Level biology – Electron microscopes

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Question 1 of 20

1. Question
The following microscope uses electrons and produces a 3D image of the surface of the specimen.
- Scanning electron microscope
- Light microscope
- Transmission electron microscope
- Mounted electron microscope
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Question 2 of 20

2. Question
The following microscope uses electrons and produces a 2D image of a thin section slicing through the specimen
- Transmission electron microscope
- Compound microscope
- Scanning electron microscope
- Simple microscope
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Question 3 of 20

3. Question
Which of these is not a limitation of electron microscopes?
- Cannot visualise a whole cell
- Cannot show colour
- Cannot image living tissue
- Artefacts might occur during sample preparation
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Question 4 of 20

4. Question
The transmission electron microscope can usefully magnify up to about:
- 1000000x
- 100000x
- 10000x
- 1000x
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Question 5 of 20

5. Question
The scanning electron microscope can usefully magnify up to about:
- 30000x
- 300000x
- 3000x
- 300x
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Question 6 of 20

6. Question
The electron beam used in an electron microscope has a wavelength that…
- Is shorter than that of visible light
- Is longer than that of visible light
- Is the same as that of visible light
- Is not related to the resolving power of these instruments
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Question 7 of 20

7. Question
Electrons used in an electron microscope are…
- Negatively charged
- Positively charged
- Have no overall charge
- The same as a photon
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Question 8 of 20

8. Question
Electromagnets can be used to focus the beam in an electon microscope owing to what?
- The negative charge of electrons
- The positive charge of electrons
- The fact that electrons have no overall charge
- Because electrons contain iron
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Question 9 of 20

9. Question
Modern electron microscopes can resolve objects that are how far apart?
- 0.1 nanometre
- 0.1 micrometre
- 0.1 picometre
- 0.1 femtometre
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Question 10 of 20

10. Question
Why do the electron beam and sample in an electron microscope need to be kept in a near vacuum?
- Because the electrons are absorbed or deflected by molecules in the air
- Because the electrons will be oxidized in the presence of oxygen
- Because dust will settle on the surface of the sample and create artefacts
- To prevent the electrons from melting the sample
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Question 11 of 20

11. Question
Which of the following best describes transmission electron microscopy?
- The beam passes through a thin section of the specimen
- The beam is directed on to the surface of specimen
- The electrons are scattered by contours on the specimen
- It has a resolving power of around 20 nm
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Question 12 of 20

12. Question
Which of the following best describes scanning electron microscopy?
- The beam is directed on to the surface of specimen
- The beam passes through a thin section of the specimen
- Dark and light parts of the image depend on the degree to which electrons are absorbed by the specimen
- It has a resolving power of 0.1 nm
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Question 13 of 20

13. Question
Which electron microscopy technique can be used to generate a 3D image of a sample?
- Both TEM and SEM
- Only SEM
- Only TEM
- Neither, electron microscopy only generates 2D images
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Question 14 of 20

14. Question
Which of the following could not be resolved with an electron microscope?
- An individual protein
- The detailed structure of an organelle
- An individual virus
- An individual ribosome
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Question 15 of 20

15. Question
Which of the following can be imaged using an electron microscope
- Only dead specimens
- Only live specimens
- Live and dead specimens
- Only inorganic specimens
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Question 16 of 20

16. Question
What is the term used to describe the image derived from an electron microscope?
- Photomicrograph
- Microphotograph
- Photomicrogram
- Microphotogram
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Question 17 of 20

17. Question
Why might details seen in the final image taken by TEM be open to doubt?
- Structures seen could be artefacts resulting from the way the specimen is prepared
- Electrons might have altered the structure of the specimen as they passed through
- Human eyes cannot resolve the details in a photomicrograph as they cannot see electrons
- Scattering of electrons by the surface of the specimen may have led to peculiarities in the image
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Question 18 of 20

18. Question
What is an artefact?
- An unnatural structure or detail seen in a photomicrograph that results from sample preparation
- An unnatural structure or detail seen in a photomicrograph that results from electrons altering the specimen as they pass through
- An unnatural object made by the microscopist
- A blemish in the image owing to collision of electrons with the beam detector
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Question 19 of 20

19. Question
Why might the 0.1 nm resolution in TEM not always be achieved in practice?
- Difficulties preparing the specimen can limit achievable resolution
- All objects being observed in some specimens might all be further apart than 0.1 nm
- The wavelength of the electron beam might increase with some samples
- Artefacts may lower the resolution
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Question 20 of 20

20. Question
What problems can be caused by the higher energy electron beam needed to achieve maximum resolution in TEM?
- The specimen may be destroyed
- The electrons may be too powerful to detect
- The electrons may lose their charge
- It may be difficult to see a stationary image with electrons at higher energies
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