GCSE Biology – Aseptic techniques

Last updated: 22/06/2020

Learning Objectives

-I can describe how to use a light microscope to observe microorganisms
-I can describe and explain the aseptic techniques used in culturing microorganisms
-I can calculate cross sectional areas of cultures and clear areas

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  1. Current
  2. Review
  3. Answered
  1. How do bacterial cells divide?

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  2. How often can bacterial cells multiply?

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  3. After one binary fission, how many cells are there?

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  4. Which factor affects rate of bacterial growth?

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  5. Which factor affects rate of bacterial growth?

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  6. How can bacteria be grown in a lab?

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  7. How can bacteria be grown in a lab?

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  8. What must our bacterial cultures be for an accurate experiment?

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  9. What is the name of the culture dish?

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  10. How do we sterilise the petri dish?

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  11. What do we call the loop we transfer bacteria with?

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  12. How do we sterilise the inoculating loop?

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  13. What does the control disc do?

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  14. What do we do with the other discs?

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  15. Why do we not fully seal the petri dish?

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  16. Why do we store the petri dish upside down?

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  17. What temperature do we incubate the dishes at?

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  18. Why do we incubate at this temperature?

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  19. Why might laboratories incubate bacteria at a higher temperature?

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  20. Which antiseptic is the best?

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  21. How do we calculate the area of the zone of inhibition?

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  22. What is the zone of inhibition?

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  23. What would happen if we incubated at 200 °C?

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  24. How could we improve the investigation?

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  25. What type of microscope is used?

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