Required practical – Separation of species by thin-layer chromatography.

Last updated: 02/11/2022

AQA A-Level Chemistry Required practicals Required practical – Separation of species by thin-layer chromatography.

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Question 1 of 25

1. Question
What compound should be added to a development chamber?
- Ethyl acetate
- Ethanoic acid
- Sodium hydroxide
- Ethanol
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Question 2 of 25

2. Question
How much ethyl acetate should be added to a development chamber?
- 10 cm $^3$
- 2 cm $^3$
- 8 cm $^3$
- 5 cm $^3$
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Question 3 of 25

3. Question
Which of the following methods can be used to analyse the composition of medicines?
- Thin-layer chromatography
- Distillation apparatus
- Refluxing
- Titrations
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Question 4 of 25

4. Question
Where should the level of solvent be within a chromatogram?
- Below the spotting line
- It does not matter
- Above the spotting line
- At the spotting line
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Question 5 of 25

5. Question
What does TLC stand for?
- Thin-layer chromatography
- Timed layered chromatograph
- Thin lasting chromatography
- Timely layered chromatography
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Question 6 of 25

6. Question
When should the TLC plate be removed from the development chamber?
- When the solvent is 1 cm from the top of the plate
- When the solvent is 0.5 cm from the top of the plate
- When the solvent is 1.5 cm from the top of the plate
- When the solvent is 2 cm from the top of the plate
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Question 7 of 25

7. Question
What is the definition of chromatography?
- A technique used to analyse and identify components with different types of crude oils
- A technique used to separating and identifying mixtures within large lattices
- A technique used for separating and identifying components in a mixture
- A technique used to analyse and separate hydrocarbons
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Question 8 of 25

8. Question
What should the plate be placed under?
- UV light
- Infared light
- Gamma radiation
- Visible light
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Question 9 of 25

9. Question
What piece of equipment should be used to crush the tablet being analysed?
- Pestle and mortar
- Spatula
- Hammer
- Spoon
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Question 10 of 25

10. Question
What should be calculated from the TLC plate?
- The Rf values
- The average length of spot
- The number of spots
- The Ra values
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Question 11 of 25

11. Question
What piece of equipment should be used to ensure efficient weighing of the tablet?
- Weighing boat
- Petri dish
- Beaker
- Pipette
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Question 12 of 25

12. Question
What type of compound is held in the development chamber?
- Solvent
- Unreactive
- Alkaline
- Water
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Question 13 of 25

13. Question
How much of each tablet should be weighed out?
- 0.1 grams
- 0.125 grams
- 0.01 grams
- 0.05 grams
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Question 14 of 25

14. Question
Which of the following is the calculation used to find the Rf value?
- Distance moved by spot / distance moved by solvent
- Distance moved by spot x distance moved by solvent
- Distance moved by solvent + distance moved by spot
- Distance moved by solvent / distance moved by spot
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Question 15 of 25

15. Question
What should each tablet be dissolved in?
- Ethanol
- Distilled water
- Hydrochloric acid
- Sodium hydroxide
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Question 16 of 25

16. Question
What should be calculated from the TLC plate?
- The distance of the spots from the line
- The Ra values
- The number of spots
- The average length of spot
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Question 17 of 25

17. Question
How much ethanol should the aspirin, ibuprofen and paracetamol tablet be dissolved in?
- 0.1 cm $^3$
- 0.5 cm $^3$
- 1.0 cm $^3$
- 7 cm $^3$
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Question 18 of 25

18. Question
What should be calculated from the TLC plate?
- The distance travelled by the solvent
- The number of spots
- The length of time taken
- The average length of spot
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Question 19 of 25

19. Question
How much ethanol should the caffeine and Anadin Extra tablet be dissolved in?
- 7 cm $^3$
- 0.5 cm $^3$
- 1.0 cm $^3$
- 0.1 cm $^3$
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Question 20 of 25

20. Question
How many tablets are to be tested using thin-layer chromatography?
- 5
- 3
- 6
- 8
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Question 21 of 25

21. Question
What should be used to draw a line on a TLC plate?
- Pencil
- Pen
- Chalk
- Ink
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Question 22 of 25

22. Question
How far up should the line be drawn on the TLC plate?
- 1 cm
- 2 cm
- 5 cm
- 10 cm
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Question 23 of 25

23. Question
How many spots should be marked on the TLC plate?
- 5
- 8
- 4
- 2
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Question 24 of 25

24. Question
What piece of equipment should be used to transfer the solution onto the TLC plate?
- Capillary tube
- Spoon
- Pipette
- Spatula
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Question 25 of 25

25. Question
What piece of equipment does a chromatograph take place on?
- Plate
- Plastic
- Cardboard
- Paper
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