TLC (Thin-Layer Chromatography) – Primrose Kitten

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TLC (Thin-Layer Chromatography)

Last updated: 26/02/2020

AQA A-Level Chemistry Amino acids, proteins and DNA TLC (Thin-Layer Chromatography)

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Question 1 of 20

1. Question
What is the definition of chromatography?
- A technique used for separating and identifying components in a mixture
- A technique used to analyse and separate hydrocarbons
- A technique used to analyse and identify components with different types of crude oils
- A technique used to separating and identifying mixtures within large lattices
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Question 2 of 20

2. Question
What is the definition of thin-layer chromatography?
- A plate is coated with a solid and a solvent moves up the plate
- A plate is coated with a liquid and a solvent moves up the plate
- A column is packed with a solid or a liquid and a gas is passed through
- A column is packed with a solid and the solvent moves the components
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Question 3 of 20

3. Question
What does TLC stand for?
- Thin-layer chromatography
- Thin-line chromatography
- Testing layer chromatography
- Textile layer chromatography
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Question 4 of 20

4. Question
What is the definition of a mobile phase?
- The carrying of soluble components within the mixture
- Holding back the components in the mixture that are attracted to it
- The movement of the components within the mixture being analysed during chromatography
- The liquid substance within chromatography
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Question 5 of 20

5. Question
What is the definition of a stationary phase?
- The carrying of soluble components within the mixture
- Holding back the components in the mixture that are attracted to it
- The movement of the components within the mixture being analysed during chromatography
- The liquid substance within chromatography
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Question 6 of 20

6. Question
Which of the following is the mobile phase within thin-layer chromatography?
- The solvent
- The pencil
- Silica gel
- Component
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Question 7 of 20

7. Question
Which of the following is the stationary phase within thin-layer chromatography?
- Silica gel
- Sodium hydroxide
- Carbon
- Water
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Question 8 of 20

8. Question
Describe the relationship between the solubility of a component in the mobile phase and the movement of the component.
- The more soluble the component in the mobile phase the faster it moves
- There is no relationship between the solubility of a component in the mobile phase and the movement of the component
- The less soluble the component in the mobile phase the faster it moves
- The more soluble the component in the mobile phase the slower it moves
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Question 9 of 20

9. Question
Describe the relationship between the affinity a component has in the stationary phase and the movement of the component.
- The more affinity the component has in the stationary phase the slower it moves
- The less affinity the component has in the stationary phase the slower it moves
- The more affinity the component has in the stationary phase the faster it move
- There is no relationship between the affinity of a component in the stationary phase and the movement of the component
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Question 10 of 20

10. Question
What is the glass coated with during thin-layer chromatography?
- Silica gel
- Sodium hydroxide
- Carbon
- Water
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Question 11 of 20

11. Question
When carrying out thin-layer chromatography where should the baseline of the glass be placed?
- Above the level of the solvent
- At the level of the solvent
- Below the level of the solvent
- In the air
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Question 12 of 20

12. Question
Which of the following can be sprayed on a chromatogram to show the colourless spots?
- Locating agent
- Acid
- Solvent
- Reducing agent
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Question 13 of 20

13. Question
How is the position of the colourless spots on the chromatogram seen?
- Shine ultraviolet light on the plate
- Shine microwave light on the plate
- View the plate under visible light
- View the plate in the dark
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Question 14 of 20

14. Question
What type of light is shone on the chromatogram?
- Ultraviolet
- Gamma
- X-ray
- Microwave
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Question 15 of 20

15. Question
How must the line on a chromatogram be drawn on?
- Using pencil
- Using paint
- Using pen
- Using chalk
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Question 16 of 20

16. Question
What is calculated from a chromatogram?
- The Rf value
- The Ra value
- The number of speed spots
- The speed of the spots
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Question 17 of 20

17. Question
What does Rf stand for?
- Retention factor
- Reform formation
- Resultant factor
- Resolvement formation
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Question 18 of 20

18. Question
Which of the following is the calculation used to find the Rf value of a component?
- Distance moved by spot / distance moved by solvent
- Distance moved by spot x distance moved by solvent
- Distance moved by solvent + distance moved by spot
- Distance moved by solvent / distance moved by spot
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Question 19 of 20

19. Question
Why are Rf values useful?
- Helps to identify each component
- Helps to identify the mixture
- Helps to tell which components are not present
- Helps to tell how many components are in a mixture
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Question 20 of 20

20. Question
Which of the following is true for all Rf values?
- All Rf values must be less than 1
- All Rf values must be more than 1
- All Rf values must be less than 2
- All Rf values are 1
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