Genome sequencing techniques

Last updated: 21/08/2021

AQA A-Level Biology Using genome projects Genome sequencing techniques

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Question 1 of 25

1. Question
DNA sequencing refers to ———
- The technique used to determine the base sequence in a DNA molecule
- The technique used to determine the sugar sequence in a DNA molecule
- The technique used to determine the phosphate sequence in a DNA molecule
- All of these
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Question 2 of 25

2. Question
The process of determining the precise order of nucleotides in the DNA is called ———–
- DNA sequencing
- DNA replication
- Denaturation
- Blotting
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Question 3 of 25

3. Question
The Chain termination method is related to ———-
- DNA sequencing
- Protein Quantification
- DNA Quantification
- Colorimetric ass
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Question 4 of 25

4. Question
Which of the following are a chemical nucleotides sequence method?
- Maxima-Gilbert Method
- Sanger method
- Edman method
- Automated sequencing method
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Question 5 of 25

5. Question
The first significant DNA sequence to be obtained was that of ________
- Lambda
- Plasmid
- Lactose
- Mammals
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Question 6 of 25

6. Question
Which of the following act as a chain terminator?
- Dideoxynucleotides
- Deoxynucleotides
- Exogenous
- DNA
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Question 7 of 25

7. Question
How many types of deoxynucleoside triphosphates are used in Sanger sequencing?
- Four
- Three
- Two
- Five
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Question 8 of 25

8. Question
What is the main enzyme component of Sanger sequencing?
- Polymerase
- Helicase
- Nuclease
- Gyrase
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Question 9 of 25

9. Question
Which of the following is not a DNA sequencing method?
- Edman method
- Maxima-Gilbert Method
- Sanger method
- LMPCR
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Question 10 of 25

10. Question
Nucleotides used to stop DNA synthesis during DNA sequencing differ from normal nucleotides in which position?
- 3'
- 1'
- 2'
- 5'
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Question 11 of 25

11. Question
DNA sequencing used in the ———–
- All of these
- Forensic studies
- Diagnostics
- Biotechnology
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Question 12 of 25

12. Question
The first DNA sequences were obtained in early ————
- 1970s
- 1960s
- 1930s
- 1920s
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Question 13 of 25

13. Question
The collection of proteins that can be produced by a given species is:
- Called the proteome
- Considered that species' genetic complement
- Correlated with the size of the organism.
- All of these
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Question 14 of 25

14. Question
The effects of protein on an entire organism are described in __________
- Phenotypic function
- Cellular function
- Molecular function
- Structural genomics
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Question 15 of 25

15. Question
The complete set of proteins expressed by an organism
- Proteome
- Considered that species' genetic complement
- Genomics
- Biotechnology
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Question 16 of 25

16. Question
The first studies of proteins that could be regarded as proteomics began in?
- 1975
- 1973
- 1974
- 1976
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Question 17 of 25

17. Question
The word proteome is a blend of the words “protein” and “genome”, and was coined by Marc Wilkins in?
- 1994
- 1993
- 1995
- 1996
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Question 18 of 25

18. Question
Expression proteomics includes the analysis of protein expression at a larger scale.
- True
- False
- May be True or False
- None of these
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Question 19 of 25

19. Question
The functions and structure of the genome is_____.
- Genomics
- species
- Inheritance
- None of these
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Question 20 of 25

20. Question
The human genome contains approximately__________.
- 3 billion base pairs
- 6 billion base pairs
- 5 billion base pairs
- 4 billion base pairs
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Question 21 of 25

21. Question
How many genes constitute the human genome?
- About 30,000
- More than 1 million
- 4 Mb
- 46
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Question 22 of 25

22. Question
The study of genome evolution is also known as ——-
- Comparative genomics
- Functional genomics
- Phylogenetic genomics
- Structural genomics
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Question 23 of 25

23. Question
Which of the following is used for determining the location of specific genes within the genome?
- Annotation
- Genomics
- Cloning
- Proteomics
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Question 24 of 25

24. Question
Studies of similarities and differences among the genomes of multiple organisms are carried out in ———
- Comparative genomics
- Proteomics
- Functional genomics
- Structural genomics
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Question 25 of 25

25. Question
Which of the following organisms has the smallest genome?
- M. genitalium
- H. influenzae
- M. tuberculosis
- None of these
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