DNA probes
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Question 1 of 25
1. Question
A lebelled DNA or RNA segment, which is used to find a specific sequence of nucelotides in a DNA molecule is named as:
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Question 2 of 25
2. Question
Long probes are usually made by:
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Question 3 of 25
3. Question
Why is a probe labelled?
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Question 4 of 25
4. Question
Which one is the basic methods used for DNA labeling?
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Question 5 of 25
5. Question
For glycoproteins, most commonly used probe is:
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Question 6 of 25
6. Question
In situ hybridization allows the use of the___________to employ in the detection of various nucleic acid present in any biological sample.
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Question 7 of 25
7. Question
The nucleic acid probes are basepairs , tagged with the coloured molecule of:
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Question 8 of 25
8. Question
The ________long complementary DNA sequence is used to detect the presence of complementary DNA sequence.
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Question 9 of 25
9. Question
A probe can be synthesised:
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Question 10 of 25
10. Question
The DNA probes are adopted for detection of the___________ present in a sample and DNA quantification.
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Question 11 of 25
11. Question
For protein detection, most commonly used probe is:
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Question 12 of 25
12. Question
The ________ are the first choice in the FISH- fluorescence in situ hybridization in which probes are allowed to hybridize directly on the chromosome.
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Question 13 of 25
13. Question
Which is the commonly used DNA probes in the realtime PCR?
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Question 14 of 25
14. Question
The chemistry of the TaqMan probe is based on the exonuclease activity of the __________ enzyme.
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Question 15 of 25
15. Question
For increasing the specificity of the reaction and results, the _______like hairpin probes are used in the quantification assay.
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Question 16 of 25
16. Question
RNA probes are often known as :
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Question 17 of 25
17. Question
It is used to detect the presence of mRNA present in the biological sample thereby detection of __________.
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Question 18 of 25
18. Question
TraditionallyRNA probe is used in the ________ hybridization in which once the RNA probes are constructed.
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Question 19 of 25
19. Question
Radio-active molecules such as________are used for the labelling of probe.
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Question 20 of 25
20. Question
The ability of individual single-stranded nucleic acid molecules to form double stranded DNA molecules is nown as:
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Question 21 of 25
21. Question
Autoradiography is used to detect the_____________.
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Question 22 of 25
22. Question
___________ binding is one of the key limitations of using DNA probes in molecular genetics.
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Question 23 of 25
23. Question
Southern blots may be used to detect the presence of certain ________________ sample.
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Question 24 of 25
24. Question
If the person has the mutant allele, the probe will bind to the complementary base sequence in one strand and can be detected using _________.
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Question 25 of 25
25. Question
One of the important application of the probe-based method is in the___________.
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