A Level Biology – Separating components

Last updated: 17/02/2022

AQA A-Level Biology Cell structure A Level Biology – Separating components

Time limit: 0

Quiz Summary

0 of 20 Questions completed

Questions:

Information

You have already completed the quiz before. Hence you can not start it again.

Quiz is loading…

You must sign in or sign up to start the quiz.

You must first complete the following:

Results

Quiz complete. Results are being recorded.

Results

0 of 20 Questions answered correctly

Your time:

Time has elapsed

You have reached 0 of 0 point(s), (0)

Earned Point(s): 0 of 0, (0)
0 Essay(s) Pending (Possible Point(s): 0)

Average score
Your score

Categories

Not categorized 0%

Current
Review
Answered
Correct
Incorrect

Question 1 of 20

1. Question
What is the process to separate cell organelles called?
- Cell fractionation
- Homogenisation
- Filtration
- Separation
Correct

Incorrect
Question 2 of 20

2. Question
During cell fractionation to separate organelles, why is the material kept on ice?
- To reduce the rate of enzymes degrading the organelles
- To help ultracentrifugation to separate the organelles
- To keep density constant
- To maintain organelle mass
Correct

Incorrect
Question 3 of 20

3. Question
To separate cell organelles, the process order should be:
- Cooling, homogenisation, ultracentrifugation
- Homogenisation, ultracentrifugation, cooling
- Cooling, ultracentrifugation, homogenisation
- Ultracentrifugation, cooling, homogenisation
Correct

Incorrect
Question 4 of 20

4. Question
What is the purpose of an isotonic buffer during cell fractionation?
- Prevents cells bursting or shrinking and prevents protein denaturation
- Prevents cells running out of energy
- Alters density of organelles so that they separate
- Maintains density of organelles so that they separate
Correct

Incorrect
Question 5 of 20

5. Question
During cell fractionation, centrifugation is done…
- At low speed and then progressively higher speeds
- At the same speed each time
- At high speed and then progressively lower speeds
- Before homogenisation
Correct

Incorrect
Question 6 of 20

6. Question
Which of the following best describes the process of cell fractionation?
- Breaking up cells and separating their organelles based on weight
- Breaking up cells
- Breaking up cells and separating organelles based on charge
- Centrifuging intact cells to separate them from growth media
Correct

Incorrect
Question 7 of 20

7. Question
Why is cell fractionation carried out in a buffered solution?
- So that the pH does not fluctuate and alter the structure of organelles or functioning of enzymes
- So that the temperature does not fluctuate and alter the structure of organelles or functioning of enzymes
- So that the cells do not dehydrate and alter the structure of organelles or functioning of enzymes
- So that the cells and organelles do not burst
Correct

Incorrect
Question 8 of 20

8. Question
Which of the following is the process by which cells are broken up?
- Homogenisation
- Cell fractionation
- Filtration
- Separation
Correct

Incorrect
Question 9 of 20

9. Question
What is the name of the machine in which cells are broken up?
- A homogeniser
- An ultracentrifuge
- An ice bath
- An electron microscope
Correct

Incorrect
Question 10 of 20

10. Question
What is the name of the fluid resulting from tissue homogenisation?
- Homogenate
- Cytoplasm
- Serum
- Filtrate
Correct

Incorrect
Question 11 of 20

11. Question
Prior to ultacentrifugation, the homogenate is treated by which process?
- Filtration to remove complete cells and large pieces of debris
- Homogenization to breakdown the organelles and release their content
- Filtration to remove organelles prior to ultracentrifugation
- Heating to 60°C to inactivate enzymes
Correct

Incorrect
Question 12 of 20

12. Question
Which of the following best describes the process of ultracentrifugation?
- Spinning tubes of homogenate at very high speed
- Passing homogenate through a filter
- Breaking up cells using a homogeniser
- Transfering supernatant from one tube to another
Correct

Incorrect
Question 13 of 20

13. Question
Which of the following best describes what occurs during centrifugation?
- The heaviest organelles are forced to the bottom of the tube
- The lightest organelles are forced to the bottom of the tube
- The heaviest organelles rise to the top of the tube
- All organelles are forced to the bottom of the tube
Correct

Incorrect
Question 14 of 20

14. Question
Put the following organelles in order of heaviest to lightest: 1. Lysosome 2. Nuclei 3. Mitochondria
- 2, 3, then 1
- 3, 2 then 1
- 1, 2 then 3
- 2, 1 then 3
Correct

Incorrect
Question 15 of 20

15. Question
What is the name of the fluid at the top of a centrifuge tube after it has been spun at high speed?
- Supernatant
- Homgenate
- Filtrate
- Buffer
Correct

Incorrect
Question 16 of 20

16. Question
Which of the following organelles would sediment during centrifugation at low speed (1000 x gravity)
- Nuclei
- Mitochondria
- Lysosome
- Ribosome
Correct

Incorrect
Question 17 of 20

17. Question
Which of the following organelles would sediment during centrifugation at medium speed (3500 x gravity)
- Mitochondria
- Lysosome
- Ribosome
- Vesicles
Correct

Incorrect
Question 18 of 20

18. Question
Which of the following organelles would only sediment by centrifugation at very high speed (100,000 x gravity)
- Ribosome
- Nuclei
- Mitochondria
- Lysosome
Correct

Incorrect
Question 19 of 20

19. Question
What is the name of the force responsible for separating organelles during cell fractionation?
- Centrifugal force
- Frictional force
- Electromagnetic force
- Strong nuclear force
Correct

Incorrect
Question 20 of 20

20. Question
During centrifugation, samples in tubes are placed in what component of a centrifuge?
- Rotor
- Shaft
- Motor
- Vacuum pump
Correct

Incorrect