A Level Biology – Separating components

Last updated: 17/02/2022
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  1. Current
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  1. What is the process to separate cell organelles called?

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  2. During cell fractionation to separate organelles, why is the material kept on ice?

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  3. To separate cell organelles, the process order should be:

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  4. What is the purpose of an isotonic buffer during cell fractionation?

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  5. During cell fractionation, centrifugation is done…

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  6. Which of the following best describes the process of cell fractionation?

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  7. Why is cell fractionation carried out in a buffered solution?

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  8. Which of the following is the process by which cells are broken up?

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  9. What is the name of the machine in which cells are broken up?

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  10. What is the name of the fluid resulting from tissue homogenisation?

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  11. Prior to ultacentrifugation, the homogenate is treated by which process?

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  12. Which of the following best describes the process of ultracentrifugation?

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  13. Which of the following best describes what occurs during centrifugation?

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  14. Put the following organelles in order of heaviest to lightest: 1. Lysosome 2. Nuclei 3. Mitochondria

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  15. What is the name of the fluid at the top of a centrifuge tube after it has been spun at high speed?

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  16. Which of the following organelles would sediment during centrifugation at low speed (1000 x gravity)

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  17. Which of the following organelles would sediment during centrifugation at medium speed (3500 x gravity)

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  18. Which of the following organelles would only sediment by centrifugation at very high speed (100,000 x gravity)

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  19. What is the name of the force responsible for separating organelles during cell fractionation?

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  20. During centrifugation, samples in tubes are placed in what component of a centrifuge?

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